Peter Cullen Optimus Prime (voice)

Jeffrey Combs Ratchet (voice)

Frank Welker Megatron (voice)

Tania Gunadi Miko Nakadai (voice)

Josh Keaton Jack Darby (voice)

Kevin Michael Richardson Bulkhead (voice)

Ernie Hudson Agent William Fowler (voice)

Andy Pessoa Rafael "Raf" Esquivel (voice)

Sumalee Montano Arcee (voice)

Nolan North Smokescreen (voice)

Steve Blum Starscream (voice)

Michael Ironside Ultra Magnus (voice)

David Sobolov Shockwave (voice)

Daran Norris Knock Out (voice)

James Horan Wheeljack (voice)

Richard Green Skyquake (voice)

Dwayne Johnson Cliffjumper (voice)

Alexandra Krosney Sierra (voice)

Clancy Brown Silas (voice)

Will Friedle Bumblebee (voice)

George Takei Alpha Trion (voice)

David Kaye Hardshell (voice)

John Noble Unicron (voice)

Peter Mensah Predaking (voice)

Tony Todd Dreadwing (voice)

Billy Brown Cliffjumper (voice)

Adam Baldwin Breakdown (voice)

Reggie Bannister Museum Security Guard (voice)

Brad Raider Vince (voice)

Gina Torres Airachnid (voice)

John DiMaggio Vogel (voice)

Robert Forster General Bryce (voice)

Markie Post June Darby (voice)